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igg2a antibody levels  (Bethyl)


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    Bethyl igg2a antibody levels
    Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and <t>IgG</t> serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).
    Igg2a Antibody Levels, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2a antibody levels/product/Bethyl
    Average 94 stars, based on 144 article reviews
    igg2a antibody levels - by Bioz Stars, 2026-03
    94/100 stars

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    1) Product Images from "Polysaccharide BAP1 of Bifidobacterium adolescentis CCDM 368 attenuates ovalbumin-induced allergy through inhibition of Th2 immunity in mice"

    Article Title: Polysaccharide BAP1 of Bifidobacterium adolescentis CCDM 368 attenuates ovalbumin-induced allergy through inhibition of Th2 immunity in mice

    Journal: bioRxiv

    doi: 10.1101/2024.09.14.613063

    Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and IgG serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).
    Figure Legend Snippet: Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and IgG serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Luminex



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    Image Search Results


    Effect of Humulus japonicas (HJ) on the production of anti-type II collagen antibody in plasma. Levels of (A) anti-type II collagen total IgG and its subtypes (B) IgG1, and (C) IgG2a were measured by ELISA in plasma obtained on day 35 from each mice group. Gene expression levels of (D) Il-12rβ2 , (E) Ccr2 , (F) Ccr5 , (G) Ccr3 , (H) Ccr4 , (I) IL-2 and (J) IL-13 were analyzed by RT-qPCR in the paw of CIA mice on day 35 following the first immunization. The vehicle group was set to a value of 1, and average fold-change is shown. Grouped quantitative data are presented as means ± SEM (vehicle group; n=8, HJ group; n=6). Significance was measured using two-tailed Student's t-test. * P<0.05, ** P<0.01, *** P<0.001.

    Journal: International Journal of Molecular Medicine

    Article Title: Humulus japonicus extract ameliorates collagen-induced arthritis in mice through regulation of overall articular inflammation

    doi: 10.3892/ijmm.2019.4417

    Figure Lengend Snippet: Effect of Humulus japonicas (HJ) on the production of anti-type II collagen antibody in plasma. Levels of (A) anti-type II collagen total IgG and its subtypes (B) IgG1, and (C) IgG2a were measured by ELISA in plasma obtained on day 35 from each mice group. Gene expression levels of (D) Il-12rβ2 , (E) Ccr2 , (F) Ccr5 , (G) Ccr3 , (H) Ccr4 , (I) IL-2 and (J) IL-13 were analyzed by RT-qPCR in the paw of CIA mice on day 35 following the first immunization. The vehicle group was set to a value of 1, and average fold-change is shown. Grouped quantitative data are presented as means ± SEM (vehicle group; n=8, HJ group; n=6). Significance was measured using two-tailed Student's t-test. * P<0.05, ** P<0.01, *** P<0.001.

    Article Snippet: Plasma samples were collected at the end of the experiment (day 35) for the determination of IgG, IgG1, and IgG2a antibody levels with three commercially available test kits, mouse anti-mouse Type II collagen IgG TMB (2036T, Chondrex), IgG1 TMB (20361T, Chondrex), and IgG2a (20362T, Chondrex) antibody subtype assay kit TMB, according to the manufacturer's instructions.

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Two Tailed Test

    Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and IgG serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).

    Journal: bioRxiv

    Article Title: Polysaccharide BAP1 of Bifidobacterium adolescentis CCDM 368 attenuates ovalbumin-induced allergy through inhibition of Th2 immunity in mice

    doi: 10.1101/2024.09.14.613063

    Figure Lengend Snippet: Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and IgG serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).

    Article Snippet: Antigen-specific IgA, IgE, IgG-Fc, IgG1, and IgG2a antibody levels were detected with Bethyl reagents (Bethyl Laboratories, Inc), according to the manufacturer’s recommendations.

    Techniques: Enzyme-linked Immunosorbent Assay, Luminex

    Levels of ovalbumin (OVA) specific immunoglobulin (Ig) E (A), OVA-specific IgG1 (B), and OVA-specific IgG2a (C) in the serum. OVA sensitization significantly increased OVA-specific IgE, OVA-specific IgG1, and OVA-specific IgG2, while intranasal treatment with CIC and PPC had no significant effect. AR, allergic rhinitis; CIC, ciclesonide; PPC, proparacaine.

    Journal: Clinical and Experimental Otorhinolaryngology

    Article Title: Effect of Proparacaine in a Mouse Model of Allergic Rhinitis

    doi: 10.21053/ceo.2017.00101

    Figure Lengend Snippet: Levels of ovalbumin (OVA) specific immunoglobulin (Ig) E (A), OVA-specific IgG1 (B), and OVA-specific IgG2a (C) in the serum. OVA sensitization significantly increased OVA-specific IgE, OVA-specific IgG1, and OVA-specific IgG2, while intranasal treatment with CIC and PPC had no significant effect. AR, allergic rhinitis; CIC, ciclesonide; PPC, proparacaine.

    Article Snippet: Anti-OVA IgG2a level was measured by Mouse Anti-OVA IgG2a antibody assay kit (Chondrex, Inc., Redmond, WA, USA).

    Techniques: